Fortgeschrittene Modul 'Molecular Protein Engineering

Organisatorial information:

Contact: Dr. Bert van Loo, b.vanloo[at], room 100.17, tel: 21632

There is a pre-meeting, see previous (general teaching) page for times. The meeting will generally take place in the commons room ("Sozialraum") at the IEB, if it is occupied please convene at Dr. Bert van Loo's office (100.17, ground floor).
The course takes place in block II (May/June) of the 'Sommer Semester', starting on the first Monday of the respective block. The total length of the course is 4 weeks with one week of holiday in the middle (Pfingstferien) when there are no lectures or practicals. Lectures take place in the mornings of the first two weeks from 10.00 (sharp) until 12.00 am and practicals are in the afternoon for the first 2 weeks, held either at Schlossplatz 4 (wetlab) or the IEB (computer excersises, room 0.15). During week 3 special lectures will be held that present specific examples of molecular protein engineering used for industrial application and research. In principle these lectures will also be held from 10.00 until 12.00, although variations may occur. The rest of the time during week 3 and 4 will be spent on the 'case study' each student will do into a 'protein engineering challenge'. The latter will be presented at the last Friday of the course.

Course objectives:

This course is an introduction into the principles of molecular protein engineering techniques and their application in biotechnological processes. Techniques that will be covered include more various mutagenesis techniques directed at a specific site within a protein, as well as more random approaches such as Directed Evolution, and strategies developped based on historical evolution such as Ancestral Sequence Reconstruction.
The course will comprise lectures on the general principles of molecular protein engineering (mutagenesis techniques, screening strategies etc..) in the first two weeks followed by some special examples in week 3.
The practicals will cover several of the mutagenesis techniques discussed during the lectures. There will also be several in silico practicals dealing with molecular docking and ancestral sequence reconstruction.
The third part of the course is a 'case study' in which each student will be given a 'protein engineering challenge'. This encompasses the design of a research strategy to achieve the goal described in the challenge using a molecular protein engineering-based approach. An example could be designing a better enzyme to be used during the synthetic process for an important pharmaceutical compound.